Coding

Part:BBa_K4651011

Designed by: Jason Gao   Group: iGEM23_DNHS-SanDiego-CA   (2023-10-10)


Imports TPA into plasmid

Importer than imports TPA into plasmid. Please see reference or citation below for more information and data.

Pardo I, Jha RK, Bermel RE, Bratti F, Gaddis M, McIntyre E, Michener W, Neidle EL, Dale T, Beckham GT, Johnson CW. Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1. Metab Eng. 2020 Nov;62:260-274. doi: 10.1016/j.ymben.2020.09.009. Epub 2020 Oct 1. PMID: 32979486.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None